{"product_id":"hormone-assays-in-biological-fluids-practical-methods","title":"Hormone Assays in Biological Fluids - Practical Methods","description":"\u003cp\u003eIn this review of Hormone Assays in Biological Fluids the bottom line is clear: this Methods in Molecular Biology volume is best for researchers and advanced students who need hands-on protocols for measuring a wide range of hormones and related steroids. The book collects step-by-step methods from experienced investigators, emphasizing noncommercial assays so laboratories can adapt or develop bespoke techniques. Our review found the greatest value in its practical tutorials on radioimmunoassay, GC-MS, HPLC and PCR and the variety of matrices covered, including blood, saliva, urine and hair.\u003c\/p\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePractical protocols:\u003c\/strong\u003e Step-by-step methods make it possible for labs to implement assays without relying solely on kits.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide hormone coverage:\u003c\/strong\u003e Procedures span glucocorticoids, aldosterone, renin activity, prolactin, melatonin and multiple androgens, allowing broad application.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMultiple biological fluids:\u003c\/strong\u003e Methods are provided for blood, plasma, urine, saliva and hair so investigators can choose the most appropriate matrix.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eComparative assays:\u003c\/strong\u003e Commercial assay procedures are described alongside noncommercial ones to aid method selection and validation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAnalytical tutorials:\u003c\/strong\u003e Clear introductions to radioimmunoassay, GC-MS, HPLC and PCR techniques help users understand underlying principles and troubleshooting.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAuthor expertise:\u003c\/strong\u003e Chapters are written by researchers who developed or applied the methods, bringing practical insight to each protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eWho It's For\u003c\/h2\u003e\n\u003cp\u003eThis volume suits laboratory scientists, clinical researchers and postgraduate students who need reproducible, adaptable assay protocols for endocrine studies. It is especially helpful for investigators setting up assays in-house or developing novel methods tailored to specific study designs, because of the emphasis on noncommercial approaches and detailed procedural steps.\u003c\/p\u003e\n\u003cp\u003eThose seeking a beginner's primer on basic endocrinology or a compact quick-reference may prefer a more introductory text; this book assumes familiarity with laboratory techniques and access to standard analytical equipment such as HPLC or GC-MS.\u003c\/p\u003e\n\u003ch2\u003ePros \u0026amp; Cons\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePros\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eComprehensive, hands-on protocols that enable in-house assay development and adaptation.\u003c\/li\u003e\n\u003cli\u003eCoverage of many hormones and matrices makes it versatile for endocrine research projects.\u003c\/li\u003e\n\u003cli\u003eTutorials on analytical methods provide background useful for troubleshooting and validation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eCons\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eNot a beginner text; readers need prior lab experience and access to analytical instruments.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003ctd\u003eTitle\u003c\/td\u003e\n\u003ctd\u003eHormone Assays in Biological Fluids (Methods in Molecular Biology, 324)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEditors\/Authors\u003c\/td\u003e\n\u003ctd\u003eMichael J. Wheeler; William D. Fraser; J. S. Morley Hutchinson\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eContent focus\u003c\/td\u003e\n\u003ctd\u003eProtocols for hormones, peptides and synthetic steroids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiological fluids\u003c\/td\u003e\n\u003ctd\u003eBlood, plasma, urine, saliva, hair\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAnalytical techniques\u003c\/td\u003e\n\u003ctd\u003eRadioimmunoassay, GC-MS, HPLC, PCR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay types\u003c\/td\u003e\n\u003ctd\u003eNoncommercial assays with commercial comparisons\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eOur Verdict\u003c\/h2\u003e\n\u003cp\u003eHormone Assays in Biological Fluids is a practical, method-focused compilation well suited to laboratories that need adaptable, validated protocols across multiple hormones and sample types. It offers good value to researchers who will implement and possibly modify assays, though it is best used by those with some prior experience and access to standard analytical equipment.\u003c\/p\u003e\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eDoes this book include protocols for saliva and hair samples?\u003c\/strong\u003e\u003cbr\u003eYes, chapters cover measurement of androgens and other hormones in saliva and hair as well as blood and urine.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAre commercial assay methods discussed?\u003c\/strong\u003e\u003cbr\u003eCommercial assays are described for comparison, but the emphasis is on noncommercial, in-house methods.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDoes it teach analytical techniques like GC-MS?\u003c\/strong\u003e\u003cbr\u003eYes, the volume contains tutorials and practical notes on GC-MS, HPLC, radioimmunoassay and PCR methods.\u003c\/p\u003e","brand":"Michael J. Wheeler, William D. Fraser, J. S. Morley Hutchinson","offers":[{"title":"Default Title","offer_id":48251181170907,"sku":"1617372994","price":90.64,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0724\/1043\/1707\/files\/61QbXPPvnBL._SL1318.jpg?v=1770931369","url":"https:\/\/gearmusthave.com\/products\/hormone-assays-in-biological-fluids-practical-methods","provider":"GearMustHave","version":"1.0","type":"link"}